H+ATPase antibody - Plasma membrane H+ATPase (rabbit antibody) AS07 260 from Agrisera

Product Specs
ItemH+ATPase antibody - Plasma membrane H+ATPase (rabbit antibody)
CompanyAgrisera
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAS07 260
Quantity50 µl
ImmunogenKLH-conjugated synthetic peptide, derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (UniProt: P20649, TAIR: At2g18960) and ATPase 2 (UniProt: P19456, TAIR: At4g30190), 3 (UniProt: P20431, TAIR: At5g57350), 4 (UniProt: Q9SU58, TAIR: At3g47950), 6 (UniProt: Q9SH76, TAIR: At2g07560), 7 (UniProt: Q9LY32, TAIR: At3g60330), 8 (UniProt: Q9M2A0, TAIR: At3g42640), 9 (UniProt: Q42556, TAIR: At1g80660), 11 (UniProt: Q9LV11, TAIR: At5g62670) of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)
ReactivityActinidia chinensis, Aesculus hippocastanum, Arabidopsis thaliana, Camellia sinensis cv. Shu-chazao, Chara australis R.Br, Chlamydmonas reinhardtii, Cucumis sativus, Cucurbita moschata, Glycine max, Kandelia obovata, Hordeum vulgare, Lolium perenne, Lycopersicon esculentum, Malus x domestica Borkh. c.v. Fuji, Marchantia polymorpha, Medicago truncatula, Nicotiana benthamiana, Nicotiana tabacum, Noccaea caerulescens, Oryza sativa, Petunia hybrida, Phalenopsis Sogo Yukidian cultivar V3, Physcomitrium patens, Picea abies, Pisum sativum, Populus tremula, Pteris vittata, Ricinus communis, Spinacia oleracea, Solanum lycopersicum, Tagetes erecta, Tetraselmis chuii, Zea mays, Vicia faba
ClonalityPolyclonal
FormatLyophilized
HostRabbit
ApplicationsImmunofluorescence, Immunolocalization, Western blot
TargetH+ATPase
Research AreaPlant Biology
PuritySerum
NCBI Gene AliasesPLASMA MEMBRANE PROTON ATPASE, OPEN STOMATA 2, AHA4, AHA6, AHA7, PMA2, AHA1, AHA2, AHA3, AHA8, AHA9, F23A5.1, F19F24_16, H(+)-ATPase 7, MJB24.16, PMA, ARABIDOPSIS THALIANA ARABIDOPSIS H(+)-ATPASE, OST2, H(+)-ATPase 11, AHA11, T8B10.1, ATAHA3, H(+)-ATPase 9, F9N11_40, H(+)-ATPase 2, H(+)-ATPase 3, H(+)-ATPase 1, H(+)-ATPase 6, F9N11.40, H(+)-ATPase 4, F9A16_7, MRG21.9, MJB24_16, H(+)-ATPase 8, F23A5_1, F9A16.7, P-TYPE H(+)-ATPASE ISOFORM 3, P-TYPE H(+)-ATPASE ISOFORM 2, MRG21_9, F19F24.16, PLASMA MEMBRANE PROTON ATPASE 2
StorageStore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Do not Store this antibody in 4°C
Molecular Weight90- 95 kDa (Arabidopsis thaliana, depending upon an isoform)

Specifications/Features

1: 1000 - 1: 5000 with standard ECL OR 1: 10 000 with ECL-Advance, enhanced chemiluminescence (WB), 1: 600 - 1: 1000 (IF), 1: 100 (IL)

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Agrisera

Box 57

Vännäs

Sweden

Phone: +46 (0) 935 33033

Email: info@agrisera.com
orders@agrisera.com

Company Profile

Website: http://www.agrisera.com

(1) Spatiotemporal deposition of cell wall polysaccharides in oat endosperm during grain developmentPlant PhysiologyOctober 20, 2023Pavani P Nadiminti, Sarah M Wilson, Allison van de Meene, Alfie Hao, John Humphries, Julian Ratcliffe, Changyu Yi, Marta Peirats-Llobet, Mathew G Lewsey, James Whelan, et al.incubated in an appropriate antimouse secondary antibody. A rabbit polyclonal H+-ATPase (Agrisera, AS07260) was used at a 1:100 dilution in combination with an Alex Fluor 568 antirabbit IgG (H+L) secondary antibody

(2) The AUX1-AFB1-CNGC14 module establishes a longitudinal root surface pH profileeLifeJuly 14, 2023Nelson B C Serre, Daša Wernerová, Pruthvi Vittal, Shiv Mani Dubey, Eva Medvecká, Adriana Jelínková, Jan Petrášek, Guido Grossmann, Matyáš Fendrychusing the antibody which recognizes multiple AHA ATPases paralogs in multiple plant species (Agrisera AS07260). To test the specificity of the antibody in Arabidopsis roots, we imaged Col-0 roots, aha2-4 mutants

(3) The COG1-OsSERL2 complex senses cold to trigger signaling network for chilling tolerance in japonica riceNature CommunicationsMay 29, 2023Changxuan Xia, Guohua Liang, Kang Chong, Yunyuan XuCat# SM-005-P). The proteins were detected by SDS-PAGE using anti-HA, anti-H+-ATPase (Agrisera, Cat# AS07260, dilution, 1:5000), anti-BiP (YOUKE, Cat# YKZP054, dilution, 1:2000), anti-actin (Huaxingbio, Cat# HX1843,

(4) The thioesterase APT1 is a bidirectional-adjustment redox sensorNature CommunicationsMay 17, 2023Tuo Ji, Lihua Zheng, Jiale Wu, Mei Duan, Qianwen Liu, Peng Liu, Chen Shen, Jinling Liu, Qinyi Ye, Jiangqi Wen, et al.(M20004 M, Abmart, China, ~27 kDa) at 1:10,000 dilution for GFP tag detection, H+-ATPase (Agrisera, AS07260, Sweden, ~95 kDa) at 1:10,000 dilution, cFBPase (Agrisera, AS04043, Sweden, ~37 kDa) at 1:10,000 dilution

(5) Resurrection of plant disease resistance proteins via helper NLR bioengineeringScience AdvancesMay 3, 2023Mauricio P. Contreras, Hsuan Pai, Muniyandi Selvaraj, AmirAli Toghani, David M. Lawson, Yasin Tumtas, Cian Duggan, Enoch Lok Him Yuen, Clare E. M. Stevenson, Adeline Harant, et al.described following SDS-PAGE. Endogenous plasma membrane ATPase was detected using an anti-H + ATPase (AS07 260) antibody (Agrisera) as a marker to show the success of membrane enrichment. Heterologous protein production

(6) An activated form of NB-ARC protein RLS1 functions with cysteine-rich receptor-like protein RMC to trigger cell death in ricePlant CommunicationsMarch 13, 2023Yiqin Wang, Zhenfeng Teng, Hua Li, Wei Wang, Fan Xu, Kai Sun, Jinfang Chu, Yangwen Qian, Gary J. Loake, Chengcai Chu, et al.membrane and soluble protein fractions. Anti-UDPase (AS05086: cytosol; Agrisera) and anti-H+-ATPase (AS07260: PM; Agrisera) antibodies were used to detect the markers indicating the cytosol and PM fractions, respectively. Co-immunoprecipitation

(7) Effector‐dependent activation and oligomerization of plant NRC class helper NLRs by sensor NLR immune receptors Rpi‐amr3 and Rpi‐amr1The EMBO JournalMarch 1, 2023Hee‐Kyung Ahn, Xiao Lin, Andrea Carolina Olave‐Achury, Lida Derevnina, Mauricio P Contreras, Jiorgos Kourelis, Chih‐Hang Wu, Sophien Kamoun, Jonathan D G JonesV5‐HRP (Sigma, V2260), HA‐HRP (Roche, 12013819001), MPK6 (Agrisera, AS12 2633), H+‐ATPase (Agrisera, AS07 260), and GFP‐HRP (Abcam, ab6663). Signals were detected using ECL substrates (Thermo Fisher, 34580). After

(8) Sensor NLR immune proteins activate oligomerization of their NRC helpers in response to plant pathogensThe EMBO JournalMarch 1, 2023Mauricio P Contreras, Hsuan Pai, Yasin Tumtas, Cian Duggan, Enoch Lok Him Yuen, Angel Vergara Cruces, Jiorgos Kourelis, Hee‐Kyung Ahn, Kim‐Teng Lee, Chih‐Hang Wu, et al.described following SDS–PAGE. Endogenous plasma membrane ATPase was detected using anti‐H + ATPase (AS07 260) antibody (Agrisera) as a marker to show the success of membrane enrichment. Author contributions Mauricio

(9) Involvement of the Membrane Nanodomain Protein, AtFlot1, in Vesicular Transport of Plasma Membrane H+-ATPase in Arabidopsis thaliana under Salt StressInternational Journal of Molecular SciencesJanuary 8, 2023Lyudmila A. Khalilova, Olga V. Lobreva, Olga I. Nedelyaeva, Igor V. Karpichev, Yurii V. BalnokinH+-ATPase derived using a number of plant proteins, as well as H+-ATPases from other organisms (cat. # AS07 260, Agrisera, Vännäs, Sweden), were used as primary antibodies. Primary antibodies were used at a 1:1000

(10) Cold‐induced calreticulin OsCRT3 conformational changes promote OsCIPK7 binding and temperature sensing in riceThe EMBO JournalJanuary 4, 2023Xiaoyu Guo, Dajian Zhang, Zhongliang Wang, Shujuan Xu, Oliver Batistič, Leonie Steinhorst, Hao Li, Yuxiang Weng, Dongtao Ren, Jörg Kudla, et al.using illuminator. Western blot was conducted using GAPDH (Sigma, SAB2701826), H+‐ATPase (Agrisera, AS07260), Bip (Agrisera, AS09481), and GFP (abcam, ab6556) antibodies, respectively. Subcellular localization For

(11) Lipid-mediated activation of plasma membrane-localized deubiquitylating enzymes modulate endosomal traffickingNat CommunNovember 12, 2022Karin Vogel, Tobias BlÃ¤ske, Marie-Kristin Nagel, Christoph Globisch, Shane Maguire, Lorenz Mattes, Christian Gude, Michael Kovermann, Karin Hauser, Christine Peter, et al.enrichment Immunoblotting was conducted using the following primary antibodies: anti-H+-ATPase (5000× diluted, Agrisera, AS07260), anti-UGPase (3000× diluted, Agrisera, AS05086), anti-Sec21 (1000× diluted, Agrisera, AS08327), anti-GFP

(12) Comprehensive analysis of glycerolipid dynamics during tobacco pollen germination and pollen tube growthFrontiers in Plant ScienceNovember 8, 2022Natalia Serrano, Přemysl Pejchar, Hana Soukupová, Martin Hubálek, Martin Potockýantibodies (Agrisera) using the recommended dilutions: Anti-H+-ATPase (Plasma membrane maker, 90-95 kDa, AS07 260), Anti-UGPase (Cytoplasm marker, 52 kDa, AS14 2813), Anti-H3 (Nuclear marker, 17 kDa, AS10 710), Anti-BiP

(13) Comprehensive Analysis of Subcellular Localization, Immune Function and Role in Bacterial wilt Disease Resistance of Solanum lycopersicum Linn. ROP Family Small GTPasesInternational Journal of Molecular SciencesAugust 27, 2022Qiong Wang, Dan Zhang, Chaochao Liu, Yuying Li, Yanni Miao(ab6556, Abcam, Cambridge, UK), anti-cAPX (AS06 180: cytosol; Agrisera, Vännäs, Sweden) and anti-H+ATPase (AS07 260: plasma membrane; Agrisera, Sweden) antibodies were used for subsequent immunoblotting analysis. For

(14) Hypoxia-Induced Aquaporins and Regulation of Redox Homeostasis by a Trans-Plasma Membrane Electron Transport System in Maize RootsAntioxidants (Basel)April 25, 2022Anne Hofmann, Stefanie Wienkoop, Sabine Lüthjefrom Agrisera (Vännäs, Sweden) and used to detect the PM-specific H+-ATPase of plants (H+-ATPase, #AS07260, 1:5000), the vacuolar H+-pyrophosphatase (V-PPase, #AS121849, 1:2500), and the mitochondrial cytochrome

(15) ENB1 encodes a cellulose synthase 5 that directs synthesis of cell wall ingrowths in maize basal endosperm transfer cellsThe Plant CellMarch 4, 2022Qun Wang, Mingmin Wang, Jian Chen, Weiwei Qi, Jinsheng Lai, Zeyang Ma, Rentao Songcat. no. H0164) was used at 1:5,000, the anti-H+ ATPase antibody (Agrisera, Vännäs, Sweden; cat. no. AS07260) was used at 1:5,000, and the anti-BIP antibody (Santa Cruz Biotechnology, Dallas, TX, USA; cat. no.

(16) Sterol Extraction from Isolated Plant Plasma Membrane Vesicles Affects H+-ATPase Activity and H+-TransportBiomoleculesDecember 16, 2021Nikita K. Lapshin, Michail S. Piotrovskii, Marina S. Trofimovacell (Bio-Rad, USA). The transfer time was 2 h at a current density of 2 mA cm−2. Primary antibodies AS07 260 (Agrisera, Vännäs, Sweden) and secondary fluorescein-labeled antibodies (Medgamal, Moscow, Russia) were

(17) Augmented CO2 tolerance by expressing a single H+-pump enables microalgal valorization of industrial flue gasNature CommunicationsDecember 1, 2021Hong Il Choi, Sung-Won Hwang, Jongrae Kim, Byeonghyeok Park, EonSeon Jin, In-Geol Choi, Sang Jun Simlight conditions (50 μE m−2 s−1). Immunoblotting The primary antibodies used were H+ ATPase (1:1,000; AS07 260) and AtpB (1:10,000; AS05 085) from Agrisera. The secondary antibody used was HRP-conjugated goat anti-rabbit

(18) Plasma Membrane Fluidity: An Environment Thermal Detector in PlantsCellsOctober 17, 2021Dora L. Cano-Ramirez, Laura Carmona-Salazar, Francisco Morales-Cedillo, Jorge Ramírez-Salcedo, Edgar B. Cahoon, Marina Gavilanes-Ruízanti-SMT1 (1:1000, Agrisera, Vännäs, Sweden, AS07 266), anti-H+-ATPase (1:10,000, Agrisera, Vännäs, Sweden, AS07 260), and anti-PsbA (1:20,000, Agrisera, Vännäs, Sweden, AS05084). 2.7. ATP Hydrolysis Assays Activity

(19) MPK6 Kinase Regulates Plasma Membrane H+-ATPase Activity in Cold AcclimationInternational Journal of Molecular SciencesJune 13, 2021Ilian Giordano Ponce-Pineda, Laura Carmona-Salazar, Mariana Saucedo-García, Dora Cano-Ramírez, Francisco Morales-Cedillo, Araceli Peña-Moral, Ángel Arturo Guevara-García, Sobeida Sánchez-Nieto, Marina Gavilanes-Ruíz(1:1000, Agrisera AS09 484), anti-SMT1 (1:1000, Agrisera AS07 266), anti-H+ -ATPase (1:10,000, Agrisera AS07 260), anti-14-3-3 proteins (1:1000, Agrisera), and anti-Phospho-p44/p42 MAPK (anti-pTEpY) (1:2000, Cell Signaling

(20) Nanoalgosomes: Introducing extracellular vesicles produced by microalgaeJournal of Extracellular VesiclesApril 27, 2021Giorgia Adamo et al.

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H+ATPase antibody - Plasma membrane H+ATPase (rabbit antibody) from Agrisera

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H+ATPase antibody - Plasma membrane H+ATPase (rabbit antibody) from Agrisera

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